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KMID : 1157520130090030139
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International Journal of Clinical Preventive Dentistry
2013 Volume.9 No. 3 p.139 ~ p.144
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Comparison of Oral Biofilm Formation on Materials of Dental Appliances
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Lee Gi-Ho
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Abstract
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Objective: Dental appliances have been used to protect teeth or oral mucosa and gingiva from danger and medicaments. However, oral appliances may provide opportunistic infection of oral pathogens by oral biofilm accumulation on dental surfaces. In this study, Biofilm formation of oral bacteria on various oral appliance materials was investigated and compared.
Methods: The resilient and rigid materials of denture appliances were used. The biofilm of Streptococcus mutans or salivary bac-teria was formed on various materials of denture appliances and then observed by confocal laser scanning microscope. The level of whole bacteria or S. mutans in the biofilm was counted by colony counting using BHI and MSB agar plate. In order to evaluate the cause of differential biofilm formation on the materials, the binding of saliva protein on the materials was investigated.
Results: The amount of S. mutans biofilm formed the least on sportquard material. Also, oral biofilm using salivary bacteria formed less on the resilient materials than the rigid materials. The binding of saliva protein on the materials is like propensity of biofilm experiments.
Conclusion: The resilient materials of denture appliances bind fewer amount than the rigid materials by which the oral bio-film may form less.
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KEYWORD
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oral biofilm, dental appliance
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